... | ... | @@ -11,4 +11,4 @@ To cope with difficult lighting situations, a high dynamic range algorithm can b |
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For microscopes with different brightfield and darkfield magnifications the current setting of the hardware must be adapted.
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Click `run` to acquire the full image. Make sure that you select the brightfield or darkfield settings of the microscope before starting the process, depending on your preferences for light or dark background. |
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Click `run` to acquire the full image. Make sure that you select the brightfield or darkfield settings of the microscope before starting the process, depending on your preferences for light or dark background. Before the image tiling is started, the maximal z-shifts are computed for all tiles. Please note: for WITec systems only small movement ranges of plus and minus 100µm are allowed per default settings. If the specimen is tilted strongly or something went wrong in adjusting the fitting points, it may lead to large movement ranges. Use a central position as a last fitting point in this case to increase the possible range, because the WITec maximum range is alway relative to the current user defined position. *Important:* make sure, that the range that is displayed before the scan starts is acceptable with the current specimen and objective lens for all tile positions. Otherwise damage of the lens is possible! If you really know what you are doing, you can increase the maximum range in the WITec control settings. |
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